Ageing, retinal pigment epithelium, protein, PEDF
the National Eye Institute (NEI) report they have discovered that the loss of the protein pigment epithelium-derived factor (PEDF) may drive age-related changes in the retina.
PEDF protects human retinal pigment epithelial (RPE) cells against oxidative stress.“PEDF Deletion Induces Senescence and Defects in Phagocytosis in the RPE,
and led by Patricia Becerra, PhD, chief of NEI’s Section of Protein Structure and Function and senior author of the study.
the expression of senescence-associated genes in the RPE of 3-month-old mice that lack the Serpinf1 gene.
In Serpinf1 deletion induced H2ax for histone H2AX protein, Cdkn1a for p21 protein, and Glb1 gene for β-galactosidase.”
We called PEDF the ‘youth’ protein because it is abundant in young retinas, but it declines during aging, IF removing PEDF leads to a host of gene changes that mimic aging in the retina.”
the RPE cell nuclei were enlarged, which may indicate changes in how the cells’ DNA is packed.
The RPE cells also had turned on four genes associated with aging and cellular senescence, and levels of the PEDF receptor were significantly below normal.
the surface of the RPE cells in the mouse lacking the PEDF protein, loop involving PEDF that maintains the levels of PEDF-R and lipid metabolism in the RPE.”
“We always wondered if loss of PEDF was driven by aging, or was driving aging,” indicates the loss of PEDF is a driver of aging-related changes in the retina.”
The PEDF loss as a cause of senescence-like changes in the RPE, highlighting PEDF as both a retinoprotective and
a regulatory protein of aging-like changes associated with defective degradation of the photoreceptor outer segment in the RPE,”